H2 competition between homoacetogenic bacteria and methanogenic archaea during biomethanation from a combined experimental-modelling approach

Competition for H2 between homoacetogenic bacteria and methanogenic archaea is commonly faced in biological biogas upgrading process reducing the potential high methane production capacities. In present work, different feeding regimes were examined using anaerobic inocula that adapted non-adapted to gaseous feedstock. Adapted inoculum could compensate increased pressure at all levels especially, produced above 95% of theoretical without accumulating acetate 0.2 atm. On contrary, accumulation was detected when a used. Microbial adaptation elevated pressures can be applied as means improve Thermodynamic analysis showed hydrogenotrophic methanogenesis had always lower permissive partial (0.43–0.85 Pa) compared homoacetogenesis (3.20–7.46 pH (neutral vs alkaline) (i.e., 0.2–2.0 atm). An unstructured kinetic model developed study influence variables hydrogenotrophic, aceticlastic pathways. The parameters proposed estimated from experimental results goodness fitting assessed by coefficient determination which indicated describes efficiently dynamics compounds involved investigated